Xenohybrid Bone Graft Containing Intrinsically Disordered Proteins Shows Enhanced In Vitro Bone Formation.




ACS Appl. Bio Mater. 2020, 3, 2263−2274


Zhu H., Gomez M., Xiao J., Perale G., Betge F., Petter Lyngstadaas S., and Haugen H. J


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ABSTRACT: Bone defects are a significant health problem worldwide, as bone is the second-most transplanted tissue after blood.
Although a myriad of bone grafts (BGs) have been used to treat bone repairs, none of them possesses all the desirable characteristics.
An approach to improve BGs is to add bio-active components, however often difficult as BG production may disrupt the biological
activities of such molecules. Here, we present a composite xenohybrid BG, SmartBonePep, with a type of biomolecule inspired by
intrinsically disordered proteins (IDPs). These synthetic peptides (named P2 and P6) are physically entrapped into the polymer
matrix of the composite BG. The effects of SmartBonePep on human osteoblasts were tested. Results showed that SmartBonePep
enhanced proliferation and osteogenic effects. In order to verify the bioactivity of P2 and P6, these peptides were tested indirectly by
being added to cell culture media too. Here, P2 or P6 exhibited promoting effects on osteogenic-related gene expressions. In this
study, we showed highly effective osteoinductive synthetic peptides P2 or P6, which possess proline-rich and intrinsically disordered
structural characters. This use of IDPs may provide promising bone enhancement biomolecules for clinical usage.


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